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Chem-Space Associates, Incorporated, was founded in 1995 by Drs. Ross Willoughby, Ed Sheehan, and Sam Mitrovich in order to develop practical tools to facilitate the growth, reliability, and efficiency of LC/MS (liquid chromatography/mass spectrometry). A Global View of LC/MS is more than the title of our book and short course; it accurately describes our companies mission. LC/MS is rapidly becoming an indispensable tool for solving many of the critical problems and answering many important questions facing our global society. We will endeavor to promote the productive use of LC/MS in all industries and in all regions of the globe. This will be done through development of LC/MS training tools, LC/MS management tools, LC/MS technologies; LC/MS products and services.

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This tutorial mainly focuses on the interpretation of mass spectra containing multiply charged molecules generated by the electrospray ionization process.

In this tutorial peptides are used to demonstrate the complexity of ESI spectra. While small molecule pharmaceutical drugs (mw 200-500) in most instances ionize quite well in electrospray they routinely display only a single charge state [M+H]1+ making interpretation fairly straight forward. Don’t be discouraged, if your business is low molecular weight analysis the majority of the principles discussed in this tutorial still apply in full scan mass analysis of small molecules.

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